Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Sequences of siRNA used to downregulate the vimentin expression

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin mRNA transcription levels in E. tenella sporozoite-infected DF-1 cells. * P < 0.05, ** < 0.01 and *** < 0.001 by Student’s t- test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin protein expression levels in E. tenella sporozoite-infected DF-1 cells. a Western blotting of the internal reference GAPDH and vimentin protein. b Gray analysis of the relative expression level of the vimentin protein. ** P < 0.01 by Student’s t -test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Expressing, Infection, Western Blot

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Total phosphorylation levels of vimentin in E. tenella sporozoite-infected DF-1 cells. a Western blotting of phosphorylated and unphosphorylated vimentin with a consistent level of total vimentin. b Gray analysis of the relative expression level of phosphorylated vimentin. * P < 0.05 and ** P < 0.01 Student’s t -test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Phospho-proteomics, Infection, Western Blot, Expressing

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Changes in vimentin distribution after E. tenella sporozoite invasion in DF-1 cells

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effect of anti-vimentin polyclonal antibody on E. tenella sporozoite invasion in DF-1 cells. ** P < 0.01 by Student’s t -test compared with anti-vimentin polyclonal antibody treatment or with the same dose of normal rabbit IgG

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effects of vimentin downregulation on sporozoite invasion in DF-1 cells. a The expression of vimentin protein in different transfection groups. b Cell growth curves of different transfection groups. c The invasion rate of sporozoites after downregulation of vimentin. ** P < 0.01 by Student’s t -test compared with downregulated vimentin expression level or the untreated group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Expressing, Transfection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Sequences of siRNA used to downregulate the vimentin expression

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin mRNA transcription levels in E. tenella sporozoite-infected DF-1 cells. * P < 0.05, ** < 0.01 and *** < 0.001 by Student’s t- test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin protein expression levels in E. tenella sporozoite-infected DF-1 cells. a Western blotting of the internal reference GAPDH and vimentin protein. b Gray analysis of the relative expression level of the vimentin protein. ** P < 0.01 by Student’s t -test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Expressing, Infection, Western Blot

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Total phosphorylation levels of vimentin in E. tenella sporozoite-infected DF-1 cells. a Western blotting of phosphorylated and unphosphorylated vimentin with a consistent level of total vimentin. b Gray analysis of the relative expression level of phosphorylated vimentin. * P < 0.05 and ** P < 0.01 Student’s t -test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Infection, Western Blot, Expressing

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Changes in vimentin distribution after E. tenella sporozoite invasion in DF-1 cells

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effect of anti-vimentin polyclonal antibody on E. tenella sporozoite invasion in DF-1 cells. ** P < 0.01 by Student’s t -test compared with anti-vimentin polyclonal antibody treatment or with the same dose of normal rabbit IgG

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effects of vimentin downregulation on sporozoite invasion in DF-1 cells. a The expression of vimentin protein in different transfection groups. b Cell growth curves of different transfection groups. c The invasion rate of sporozoites after downregulation of vimentin. ** P < 0.01 by Student’s t -test compared with downregulated vimentin expression level or the untreated group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Expressing, Transfection